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Image Search Results
Journal: Frontiers in Immunology
Article Title: Polymorphisms in STING Affect Human Innate Immune Responses to Poxviruses
doi: 10.3389/fimmu.2020.567348
Figure Lengend Snippet: Phosphorylation of IRF3 and TBK1 is delayed and decreased in the presence of H232 STING. (A) STING pathway activation (phosphorylation of IRF3 and TBK1) after 2’3’ cGAMP stimulation of HEK 293 T cells, transiently expressing WT or H232 STING variants/alleles and cGAS. (B) STING pathway activation (phosphorylation of IRF3 and TBK1) after 2’3’ cGAMP stimulation of lentivirus-created BJAB cell lines, stably expressing WT or H232 STING variants under Blasticidin selection. Each experiment was conducted three times with two biological duplicates per sample.
Article Snippet: BJAB cell lines, each expressing one of the rs1131769 variants of interest, were created using custom suCMV promoter-based lentivectors containing a
Techniques: Phospho-proteomics, Activation Assay, Expressing, Stable Transfection, Selection